FIELD: medicine. SUBSTANCE: method comprises the steps of adding 96 deg ethyl alcohol cooled to 4 C in a 1: 8 proportion to sample of blood serum, incubating the mixture at 4 C for 15 min, centrifuging it for 10 min at 1000 rpm, collecting and drying supernatant liquid, dissolving dry residue in physiological solution exceeding three times the initial volume of serum, centrifuging the solution at 1000 rpm for 10 min, then placing obtained supernatant in the form of drops 0.02 ml each on object glass followed by adding 0.01 ml of physiological solution to 1st drop, 0.01 ml of Deltasone peptide in terminal concentration of 0.05 mg/ml to second drop and dalargin in a final concentration of 0.2 mg/ml to third drop. Then, 0.01 ml of cell culture of paramecium caudatum is added additionally to each drop to determine the time of paramecium reaction and, if this parameter in two last drops deviates from its value in first drop, the prognosis is unfavorable progress of ischemic heart disease. EFFECT: higher efficiency. 2 tbl
Authors
Dates
1994-01-15—Published
1989-12-11—Filed