METHOD OF PEROXIDASE HYDROLYSATE TREATMENT FOR PHENOL ASSAY Russian patent published in 1995 - IPC

Abstract RU 2037521 C1

FIELD: biochemistry. SUBSTANCE: method involves preparing horseradish peroxidase hydrolysate, and hydrolysis is carried out in 1N HCl for 0.5-6 h without evaporation under vacuum, then hydrolysate is alkalinized up to pH 10.0 and neutralized with 1N HCl. Proposed method ensures to split native complex enzyme peroxidase + phenols (quinones). EFFECT: simplified method for quinone conversion to free phenols, diminished time for hydrolysis. 1 tbl

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RU 2 037 521 C1

Authors

Andreeva V.A.

Koren' O.G.

Dates

1995-06-19Published

1992-05-13Filed