METHOD OF SULFATED GLYCOSEAMINOGLYCANS PREPARING Russian patent published in 1998 - IPC

FIELD: biotechnology. SUBSTANCE: agriculture animal corneas are placed in a mixture of equal parts of physiological solution and distilled water at ratio to cornea tissue and an aqueous mixture 1:1, frozen three-fold at 0 C, milled to particle size 0.1 x 0.1 mm and ethanol is added to final concentration 1%. Mixture is homogenized, ethanol is added again to final concentration 5% and boiled for 5-10 min. Method provides preparing the end product at high content of sulfated glycoseaminoglycans and practically no containing protein and other inert substances. EFFECT: improved method of preparing. 1 ex