FIELD: biotechnology, in particular, production of eubiotic preparations. SUBSTANCE: method involves preparing ferment by cultivating bifidobacteria of Bifidibacterium bifidum, Bifidobacterium longum, Bifidobactrium infantis or Bifidobacterium adolescentis kinds in nutritive medium; introducing protective medium into it with following liophylic drying or preparing cultured milk product in liquid state by introducing sown dose of obtained ferment into pasteurized milk in an amount not exceeding 5% of volume of nutritive medium; providing repeated cultivation of mentioned kinds of bifidobacteria at temperature of 38 C plus and minus 1 C till bunching; cooling and packing product. To produce ferment or cultured milk product in liquid state, each kind of bifidobacteria is separately cultivated till obtaining of biomasses titrated to at least 109 CFU/ml and joined together prior to drying of ferment or prior to packing of biomass of cultivated milk product of bifidobacteria to obtain product in the form of mixture of mentioned kinds of bifidobacteria. To produce preparation for babies of up to 3-5 years, bifidobacteria of Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium breve or Bifidobacterium infantis kinds are used, and for teenagers or grownups - bifidobacteria of Bifidobacretium bifidum, Bifidobacterium longum and Bifidobacterium adolescentis kinds are used. Ratio of biomass of separate kinds of bifidobacteria in preparation is set in accordance with ratio of these bifidobacteria in intestines of healthy individual of definite age, obtained on the base of statistic data and regression analysis. EFFECT: improved medicinal and prophylactic properties of bifidopreparations, wider range of usage, improved compatibility with individual intestines normoflora and simplified production method by using optimum kinds of bifidobacteria in preparation. 4 cl, 1 dwg, 2 tbl, 6 ex
