CULTURAL MEDIUM FOR PREPARING ENZYME CELLULASE IN ITS INDUSTRIAL PRODUCTION BY SUBMERGED CULTURING FUNGUS TRICHODERMA VIRIDE 44-11-62/3 AND METHOD OF PREPARING ENZYME CELLULASE ON THIS MEDIUM Russian patent published in 2001 - IPC

Abstract RU 2165974 C2

FIELD: biotechnology, biochemistry, microbiology. SUBSTANCE: invention relates to industrial production of cellulase. Method involves submerged culturing fungus Trichoderma viride 44-11-62/3 on medium containing corn starch and wheat bran hydrolysates as the source of carbon. Fermentation process is carried out at discrete addition of 0.5% solution of lactose as an activating addition. Use of homogeneous nutrient medium ensures to exclude mechanical loss in production of enzyme. Method provides attainment of increased activity of cellulase in cultural broth (27 U/ml) and increased yield of enzyme preparation celloviridine by 24%. EFFECT: improved method of enzyme producing, increased yields. 2 cl, 2 ex

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RU 2 165 974 C2

Authors

Vasil'Ev A.E.

Ogorel'Tsev B.D.

Safonov V.S.

Agafonov E.L.

Devet'Jarova L.S.

Dates

2001-04-27Published

1999-05-14Filed