FIELD: microbiology, biochemistry, molecular biology, biotechnology. SUBSTANCE: invention relates to a method for preparing amino acids L-glutamine, L-arginine, L-tryptophan, L-histidine and L-glutamic acid that involves culturing microorganism belonging to genus Escherichia transformed with DNA fragment encoding mutant glutamine synthetase wherein tyrosine amino acid residue corresponding to the position 397 in the natural glutamine synthetase is replaced with residue of another amino acid, preferably with phenylalanine. The strain of Escherichia coli VL33thrC+/pMWglnAphe-4 can be used as a strain-producer. EFFECT: valuable properties of strain. 9 cl, 1 tbl, 1 dwg, 3 ex
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