FIELD: biotechnology.
SUBSTANCE: L-amino acids, such as L-glutamic acid, L-proline and L-leucine are obtained by cultivation of bacteria belonging to genus ESCHERICHIA with inactivated gadB gene encoding glutamate decarboxylase isozyme. As producer strains E.coli VL 334 thrC+ Δ gadB::cat, E.coli 702ilvA Δ gadB::cat, E.coli 57/pMWleuA 479-Δ gadB::cat are used.
EFFECT: L-amino acid with high activity.
13 cl, 2 dwg, 3 tbl, 4 ex
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