METHOD OF DETERMINING GLYCINE IN AN AQUEOUS SOLUTION Russian patent published in 2006 - IPC C07C227/38 C07C229/06 

FIELD: analytical methods.

SUBSTANCE: method of determining glycine when developing continuous protein fermentation processes consisting in preparing aqueous solution of glycine followed by extraction and further analysis of organic phase is characterized by that 20-35% by weight of lithium sulfate as salting-out agent is added to aqueous solution of glycine (pH˜5) and then solution is extracted with ternary mixture of hydrophilic solvents: 65-65.5% isopropyl alcohol, 20-20.5% acetone, and 14-15% ethyl acetate at volume ratio of extractant mixture to aqueous salt solution 1:10. Nonaqueous concentrate is further analyzed using potentiometric titration technique.

EFFECT: increased degree of glycine recovery to 98-99% and avoided use of dangerous reagents in organic phase analysis.

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