METHOD FOR PRODUCTION OF ALPHA-FETOPROTEIN Russian patent published in 2007 - IPC A61K38/17 A61K38/02 A61K47/40 

FIELD: chemical and pharmaceutical industry, in particular method for production of alpha-fetoprotein (AFP).

SUBSTANCE: claimed method includes target preparation isolating and from blood serum and purification by double affinity chromatography, wherein between chromatography purification steps proteins are fractionated with 0.1-1 g/l aprothynine and 19-40 % ethanol at pH 5.7-5.8 and at temperature from -3°C to -5°C, and inactivation of bacteria and viruses is carried out before the first purification step on immunosorbent in presence of chloroform and Triton X-100 solutions. Blood serum is applied on affinity sorbent with preliminary blockage of non-specific sorption active site with cattle serum, and application on immunoaffinity sorbent with antibodies against AFP is carried out at pH 8.0-9.0 at 18-20°C. Impurity proteins of blood serum are removed by non-specific elution with 1 M and 2 M magnesium chloride solution, and affinity-bonded AFP is eluted with 4 M magnesium chloride solution and Gly-HCl buffer solution with pH 2.0-3.0, and as the second affinity sorbent sefarose with antibodies against human IgG is used.

EFFECT: new method for production of alpha-fetoprotein.

1 ex, 1 tbl, 1 dwg