METHOD FOR PREPARING AND CLEANING PLACENTAL ALKALINE PHOSPHATASE Russian patent published in 2013 - IPC A61K35/50 A61K38/17 A61K38/46 C12N9/16 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, namely a method for preparing placental alkaline phosphatase. A method for preparing placental alkaline phosphatase consists in the fact that blood-washed human placenta is homogenised; the homogenate is extracted in a solution of potassium chloride; the extract was centrifuged, heated, centrifuged; after the extract is primarily heated, the supernatant is heated again in the presence of cadmium sulphate; the flask is quickly cooled; ammonium sulphate is added; the precipitate is separated by centrifugation; the supernatant is subjected to hydrophobic column chromatography, and a ferment eluate is cleaned by preparative polyacrylamide gel electrophoresis; the ferment gel is coloured; the alkaline phosphatase fractions are sectioned under direct vision.

EFFECT: method enables simplifying the process of preparing and cleaning the placental alkaline phosphatase enzyme with a significant yield increase.

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