FIELD: biotechnology.
SUBSTANCE: at first, gel filtration of the solution of Central Asian cobra venom (Naja naja oxiana) is carried out on superfine Sephadex G-75, followed by chromatography of endonuclease on SP-Sephadex C-25, dialysis of the isolated enzyme with the addition of ballast protein, sterilisation and lyophilisation. At that prior to dialysis BSA is added to the final concentration of 1.0-2.0 mg/ml, and the dialysed endonuclease without concentration is sterilised and lyophilised.
EFFECT: method enables to increase the yield of endonuclease while isolation, as well as to eliminate the obstacle to its use for treatment of cow rabies.
2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR ENDONUCLEASE CONCENTRATION FROM COBRA VENOM | 0 |
|
SU1721087A1 |
| METHOD FOR ISOLATION OF TWO-ENZYME BIOCATALYST FOR PRODUCTION OF NUCLEOSIDES | 0 |
|
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| METHOD OF PRODUCTION OF PLASMINOGEN ACTIVATOR | 2007 |
|
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| METHOD OF ISOLATING ACETYLCHOLINE ESTERASES | 0 |
|
SU721450A1 |
| METHOD FOR ISOLATING OXYDASE OF L-AMINO ACIDS FROM POISON OF VIPERA LEBETINA | 0 |
|
SU1055769A1 |
| METHOD FOR PREPARATION OF C@/M@-DEPENDANT ENDONUCLEASE INHIBITOR | 0 |
|
SU1763488A1 |
| METHOD OF PRODUCING PHOSPHOESTHERASES OF PENICILLIUM BREVICOMPACEUM FUNGI | 0 |
|
SU1402619A1 |
| DIRECT THROMBIN INHIBITOR, POSSESSING ANTIPROLIFERATIVE ACTION | 2007 |
|
RU2369615C2 |
| PROCESS FOR PREPARING RNA-LIGASE | 0 |
|
SU910762A1 |
| METHOD OF RECOVERING GROWTH FACTOR OF NERVE TISSUE FROM SNAKE POISON | 0 |
|
SU1055732A1 |
