FIELD: biotechnologies.
SUBSTANCE: in the water reservoir they sample larvae of chironomids of IV stage of development with their subsequent fixation and preparation of temporary cytological preparations of polytene chromosomes of salivary glands in larvae according to aceto-orcein methodology. Extent of water reservoir contamination is decided upon according to condition of polytene chromosomes and chromosomal indices.
EFFECT: method simplification with simultaneous increase of accuracy in detection of indices of chironomids population polymorphism indices in water ecosystems.
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