FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is a method which involves preparing a protective medium (sucrose-gelatine containing 10 % sucrose and 2 % gelatine); growing indicator strain Bacillus anthracis STI-1 for propagation of anthrax bacteriophage; obtaining a concentrated suspension of an anthrax bacteriophage by a double-layer agar method, by extracting negative colonies from an upper, semi-liquid protective agar, a sucrose-gelatinous medium; preparing ion-exchange resin in penicillin bottles; sparing drying (dehydration) by applying a concentrated suspension of anthrax bacteriophage in a protective medium at temperature 0 °C to sorbent - ion-exchange resin of KB-4P-2 grade, dried to weight fraction of moisture of not more than 1÷3 % at optimum ratio of suspension of bacteriophage and resin -1:7; storage of dried cultures of anthrax bacteriophage in penicillin bottles at temperature 2÷6 °C.
EFFECT: declared method allows long-term storage of cultures of anthrax bacteriophages and their use after resuspension by resuspension at temperature 0°C in meat-peptone broth (MPB) for obtaining diagnostic preparations used for indication and identification of anthrax agent and in other areas of microbiology.
1 cl, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DRY PROBIOTIC PREPARATION AND METHOD FOR ITS PREPARING | 2003 |
|
RU2268926C2 |
| METHOD OF PRESERVATION OF MICROORGANISMS | 0 |
|
SU1730138A1 |
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| METHOD FOR PRODUCING PATHOGENIC BIOLOGICAL AGENT SIMULATORS | 2015 |
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| METHOD OF CONTACT-SORBTION DEHYDRATATION OF HIGH-DISPERSE BIOLOGICALLY ACTIVE MATERIALS | 2009 |
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RU2455349C2 |
| Bacillus anthracis R/D BATERIOPHAGE STRAIN USED TO PRODUCE PREPARATION FOR ANTHRAX INFECTION DIAGNOSTICS, LIQUID PREPARATION FOR ANTHRAX INFECTION DIAGNOSTICS AND PREPARATION FOR ANTHRAX INFECTION DIAGNOSTICS | 2007 |
|
RU2351650C1 |
