FIELD: microbiology.
SUBSTANCE: disclosed is a method for express diagnostics of biocontamination of basalt-plastic reinforcement (BPR) by cryophilic microorganisms, consisting in determining the presence or absence of cells of cryophilic microorganisms in the washing liquid taken from the surface of the BPA rods during exposure thereof under conditions of extremely low ambient temperatures. BPA rods or fragments thereof exposed under conditions of extremely low ambient temperatures from 0 to minus 42°C are used thereon as an object for express diagnostics of biocontamination; the washing liquid taken from the surface of the examined BPA rods is stained with a luminescent dye, followed by preparing a microscopic preparation and examining the microscopic preparation in a fluorescent microscope.
EFFECT: invention ensures express diagnostics of biocontamination of BPR by cryophilic microorganisms during exposure in conditions of an open ecosystem at extremely low ambient temperatures, which is relevant during operation of the BPA in the natural climatic conditions of the Far North.
1 cl, 2 tbl, 1 ex
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Authors
Dates
2021-10-11—Published
2020-10-20—Filed