FIELD: biotechnology.
SUBSTANCE: methods and compositions for cell transfection using plasmids are described. In certain implementations, methods and compositions are described, in which the transfection efficiency is significantly increased by bringing transduced cells into contact with polyethyleneimine (PEI) that does not contain nucleic acid during transfection. Therapeutically applicable vectors based on adeno-associated viruses are also described, obtained using described methods and compositions.
EFFECT: invention expands the arsenal of therapeutic means.
58 cl, 6 dwg, 2 tbl, 5 ex
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