METHOD FOR OBTAINING BIOMASS OF MICROALGAE CHLORELLA VULGARIS Russian patent published in 2023 - IPC A01G33/00 C12N1/12 

FIELD: biotechnology.

SUBSTANCE: invention relates to methods for obtaining biomass of Chlorella vulgaris microalgae. According to the method Chlorella vulgaris microalgae is placed in a nutrient medium containing a set of macro- and microelements at a temperature of 35–37°C and pH=5.5–6.5. After 72 hours of exposure, the microalgae dispersion is separated and crushed by disintegrating microalgae cells by exposure to ultrasonic radiation with the following parameter values: working solution sonication frequency is 40–60 kHz, ultrasonic radiation modulation frequency is 70–100 Hz, working solution sonication time is 23–60 minutes, relative power of ultrasonic radiation is 151–300 W/l. Then the biomass of microalgae is dried at a temperature of 40°C within 12 hours.

EFFECT: obtaining a biomass of microalgae Chlorella vulgaris with a size of individual sample cells of the order of 0.6–1.2 microns and an antioxidant activity of a biomass sample of 87 mg/l in terms of gallic acid, which will find its application in the production of feed additives, vitamin and mineral premixes for animal husbandry, as well as in the food industry for the production of functional additives for food enrichment.

1 cl, 4 dwg