METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT OF rs5762821 (G>A) OF HUMAN LONG NON-CODING RNA GENE (LNCRNA, ENSEMBL ID: ENSG00000226471) BY REAL-TIME POLYMERASE CHAIN REACTION Russian patent published in 2024 - IPC C12Q1/68 C12Q1/6806 C12Q1/6827 

Abstract RU 2825465 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a method for genotyping a single nucleotide variant of rs5762821 (G>A) of the gene of long non-coding human RNA ENSG00000226471. Said method is carried out by real-time polymerase chain reaction using forward and reverse primers lncRNA, as well as lncRNA-G-allele-specific and lncRNA-A-allele-specific fluorescent-labelled probes.

EFFECT: invention provides an effective method for genotyping a single-nucleotide variant of rs5762821 (G>A) of the gene of long non-coding human RNA by real-time polymerase chain reaction.

1 cl, 2 dwg, 3 ex

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RU 2 825 465 C1

Authors

Postnikova Mariia Igorevna

Azarova Iuliia Eduardovna

Klesova Elena Iurevna

Polonikov Aleksei Valerevich

Dates

2024-08-26Published

2024-01-23Filed