FIELD: biotechnology. SUBSTANCE: vector pPS7 (size is 8.5 thousand base pairs) is constructed, and it is inherited constantly in bacteria Pseudomonas. Vector determines the resistance to kanamycin and tetracycline, it has 8 unique sites for restriction endonucleases, it is mobilized with conjugating plasmids RP4Kms and pRP 2013. Method of this vector preparing involves insertion of par-locus of pMT2 plasmid into pPS5 plasmid. EFFECT: preparing of vector indicated above. 3 cl, 2 dwg, 1 tbl
Authors
Dates
1994-05-30—Published
1991-06-17—Filed