FIELD: gene engineering, in particular production of useful metabolites.
SUBSTANCE: Mob-derived RSF1010 plasmid contains RSF1010-replicon and as selective marker it contains thymidilate syntase gene instead of antibiotic-resistance gene. Cultivation of bacteria containing said plasmid makes it possible to produce useful metabolites.
EFFECT: safe and ecologically friendly method.
14 cl, 5 dwg, 2 tbl, 12 ex
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