FIELD: biotechnology. SUBSTANCE: method involves homogenization of common wormwood callus culture, extraction of enzyme with aqueous 0.5-2.0M solution of NaCl, fractionation of extract with ammonium sulfate, ion-exchange chromatography on DEAE-cellulose and CM-cellulose and gel-filtration on Sephadex Toyopearl-50. EFFECT: improved method of peroxidase preparing. 1 dwg
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Authors
Dates
1995-03-27—Published
1992-01-08—Filed