METHOD OF PHOSPHOLIPASE D ACTIVITY ASSAY Russian patent published in 1995 - IPC

Abstract RU 2035719 C1

FIELD: biochemistry. SUBSTANCE: phospholipase D activity is determined by phosphatidic acid quantity formed after enzymatic hydrolysis of phosphatidylcholine with phospholipase D. Phosphatidylcholine hydrolysis is performed by treatment of samples with dye Victoria Blua R in aqueous saturated solution of urea. Stained complex of phosphatidic acid is separated for phases. Quantity of phosphatidic acid formed is determined by spectrophotometry method in organic phase. EFFECT: improved method of assay. 1 dwg

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RU 2 035 719 C1

Authors

Eremin V.A.

Janopol'Skaja N.D.

Dates

1995-05-20—Published

1992-03-13—Filed