FIELD: medicinal biotechnology. SUBSTANCE: method involves construction of recombinant plasmid DNA carrying species-specific fragment of anthrax plasmid pXOI which is used for DNA-probe. Synthesis level of recombinant plasmid in cells of strain is 1.5-2 mg per 1 l nutrient medium. DNA-probe prepared on the basis of plasmid DNA pZAT1 is hybridized only with toxigenic strains of anthrax pathogen that ensures to differentiate their from other bacteria including closely related bacilli and avirulent strains Bacillus anthracis no containing plasmid pXOI. EFFECT: construction of plasmid DNA indicated above. 4 cl, 1 dwg, 1 tbl
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PRO PF5 DETERMINING SYNTHESIS OF CAPSULE ANTIGEN FI OF PLAGUE PATHOGEN AND PROTECTIVE ANTIGEN OF ANTHRAX PATHOGEN AND A METHOD OF CONSTRUCTION OF RECOMBINANT PLASMID DNA PRO PF5 |
1995 |
|
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| WAY OF RECEPTION FUNCTIONALLY ACTIVE RECOMBINANT PROTEIN OF ULCER (LF) LETHAL FACTOR, RECOMBINANT PLASMIDE DNA pETHIS-LF CODING ACTIVE PROTEIN LF; H STRAIN ESCHERICHIA COLI BL-HISLF PRODUCING ACTIVE PROTEIN OF MALIGNANT ANTHRAX LETHAL FACTOR |
2007 |
- Kolesnikov Aleksandr Vladimirovich
- Zakharova Marija Jur'Evna
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- Shemjakin Igor' Georgievich
|
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| RECOMBINANT PLASMIDE DNA FOR INDENTIFICATION OF STRAIN OF PLAGUE MICROBE |
0 |
- Bulgakova Elena Germanovna
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|
SU1615181A1 |
| METHOD OF PRODUCING FUNCTIONALLY ACTIVE RECOMBINANT PROTEIN OF LETHAL FACTOR ANTHRAX (LF), RECOMBINANT PLASMID DNA pETGST-LFmin, ENCODING ACTIVE LF PROTEIN AND STRAIN OF Escherichia coli BL-GSTLFmin, PRODUCING ACTIVE PROTEIN OF LETHAL FACTOR ANTHRAX |
2007 |
- Kolesnikov Aleksandr Vladimirovich
- Zakharova Marija Jur'Evna
- Kozyr' Arina Vladimirovna
- Shemjakin Igor' Georgievich
|
RU2355769C1 |
| RECOMBINANT PLASMID DNA PRD100 - A SOURCE OF PROBE FOR IDENTIFICATION OF PLAGUE PATHOGENE, METHOD OF ITS CONSTRUCTION, AND STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF RECOMBINANT PLASMID PRD100 |
0 |
- Podladchikova Olga Nikolaevna
- Mishankin Boris Nikolaevich
|
SU1677059A1 |
| RECOMBINANT PLASMID DNA P VR6-1 THE SIZE OF 3406 NUCLEOTIDE PAIRS USED FOR PREPARING SPECIFIC DNA-PROBE FOR HUMAN ROTAVIRUS 1-ST SUBGROUP DETECTION AND THE STRAIN OF TRANSFORMED MICROORGANISM ESCHERICHIA COLI USED FOR PREPARING RECOMBINANT PLASMID DNA P VR6-1 AND SPECIFIC PROBE FOR HUMAN ROTAVIRUS 1-ST SUBGROUP DETECTION |
1992 |
- Novikova N.A.
- Epifanova N.V.
|
RU2064502C1 |
| METHOD FOR DETECTION OF SWINE PARVOVIRUS, RECOMBINANT PLASMID DNA PPV4, USED AS A HYBRIDIZATIONAL PROBE FOR SWINE PARVOVIRUS DETECTION, AND RECOMBINANT PLASMED DNA M13, USED AS A HYBRIDIZATIONAL PROBE FOR IDENTIFICATION OF SWINE PARVOVIRUSES |
0 |
- Koromyslov Georgij Fedorovich
- Artyushin Sergej Konstantinovich
- Zaberezhnyj Aleksej Dmitrievich
|
SU1664831A1 |
| RECOMBINANT PLASMID DNA PBANP 464 ENCODING METALLOPROTEINASE OF BACILLUS AMYLOLIQUEFACIENS A-50, AND STRAIN OF BACTERIUM BACILLUS AMYLOLIQUEFACIENS - A PRODUCER OF METALLOPROTEINASE |
1989 |
- Jomantas Ju.V.
- Gervinskas V.V.
- Naroditskaja V.A.
- Kozlov Ju.I.
- Ulanova T.I.
- Sterkin V.Eh.
- Strongin A.Ja.
- Izotova L.S.
- Debabov V.G.
|
RU1679800C |
| RECOMBINANT PLASMID DNA "pДТИ-23", METHOD OF PREPARING RECOMBINANT PLASMID DNA "pДТИ-23" AND STRAIN OF BACTERIUM ESCHERICHIA COLI CONTAINING RECOMBINANT PLASMID DNA "pДТИ-23" - A PRODUCER OF THE FUSED PROTEIN DTI-2 |
1993 |
- Shemjakin I.G.
- Anisimova V.A.
- Mitrofanova G.N.
|
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| RECOMBINANT PLASMID DNA PG21 ENCODING HUMAN β-INTERFERON IN PLANTS |
1993 |
|
RU2035513C1 |
