FIELD: biotechnology. SUBSTANCE: recombinant plasmid DNA pG21 carrying chromosomal gene copy together with sequence encoding transient peptide is reconstructed. Method ensures to prepare plant showing resistance to viral infection. Proposed plasmid has a size 9.1 thousand nucleotide pairs (tnp) and consists of the following fragments: DNA vector plasmid PGS H 160, size is 8.5 tnp, HindIII-Bam HI-fragment of DNA that is a fragment of human b-interferon chromosomal gene; gene bla encoding b-lactamase; gene Sm/Sp encoding resistance to streptomycin and spectomycin; gene NP, TII encoding neomycin phosphotransferase II providing resistance of transgenic plants to kanamycin, and promoters PT1 and PT2. Proposed recombinant plasmid DNA pG21 encodes synthesis of human b-interferon in plants that ensures to prepare plants showing resistance to viral infection. EFFECT: preparing plasmid indicated above. 4 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA PG11 ENCODING SYNTHESIS OF HUMAN BETA-INTERFERON IN TRANSGENIC PLANTS AND A METHOD OF PRODUCING TRANSGENIC PLANTS SHOWING RESISTANCE TO VIRAL INFECTION | 1994 |
|
RU2103361C1 |
| RECOMBINANT PLASMID DNA pBi101-IL18 ENCODING HUMAN INTERLEUKIN-18 SYNTHESIS IN TRANSGENIC PLANTS | 2005 |
|
RU2302460C1 |
| RECOMBINANT PLASMID pBi121-ESAT6-CFP10-gIFN, CODING PROTEINS ESAT6 AND CFP10 MYCOBACTERIUM TUBERCULOSIS AND HUMAN GAMMA-INTERFERON IN TRANSGENIC PLANTS | 2011 |
|
RU2468082C1 |
| PROCESS OF OBTAINING TRANSGENE TOBACCO PLANTS WITH INCREAED CONTENT OF PROLYL | 2006 |
|
RU2324737C1 |
| PROCESS OF OBTAINING TRANSGENIC TOBACCO PLANTS WITH INCREAED CONTENT OF PROLINE | 2006 |
|
RU2324736C1 |
| METHOD OF GROWING TRANSGENIC CARROT PLANTS, WHICH PRODUCE HUMAN INTERLEUKIN-10 | 2007 |
|
RU2374321C2 |
| METHOD FOR PREPARING TRANSGENIC PLANTS RESISTANT TO VIRAL INFECTION | 2005 |
|
RU2300567C1 |
| RECOMBINANT PLASMID, PROVIDING FOR EXPRESSION OF GENE OF EXTRACELL RIBONUCLEASE Zinnia elegans ZRNaseII IN TRANSGENIC PLANTS (VERSIONS) AND METHOD FOR PRODUCTION OF VIRUS-RESISTANT FORMS OF PLANTS | 2009 |
|
RU2393226C1 |
| RECOMBINANT PLASMID DNA pBinPLUS-ARS-EPSPS ENABLING GENE EXPRESSION OF AGROBACTERIAL 5-ENOLPIRUVOYL-SHIKIMATE-3-PHOSPHAT-SYNTHETASE IN TRANSGENE CHLORELLA MICROALGAE | 2009 |
|
RU2407794C1 |
| METHOD OF TRANSGENE PLANT PREPARING, RECOMBINANT PLASMID DNA PTI VY4 RESERVED FOR PREPARING OF TRANSGENE PLANTS, AND A STRAIN OF BACTERIUM AGROBACTERIUM TUMEFACIENS USED FOR PLANT TRANSFORMATION | 1988 |
|
SU1547316A3 |
