FIELD: oil industry. SUBSTANCE: proposed biopreparation consists of the highly active living aerobic oil-oxidizing bacterium (Mycobacterium, Pseudomonas etc.) grown on the liquid nutrient medium at titer 2,5-7•107 cell/ml depending on genus and species of bacteria used. Ammonium oxalate (0.05-1.0%) and normal paraffins (1.0-1.5%) were added additionally to the liquid preparation to induce oil-oxidizing activity and high titers attainment. Biopreparation does not show pathogenicity, does not pollute environment, useful life at 6-12 C is up to 1 month. Utilization degree of diesel fuel in soil is 73-94%, on water surface is 100% using proposed biopreparation. EFFECT: simplified method of preparing, decreased cost, high effectiveness of biopreparation. 3 tbl
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