STRAIN OF BACTERIA BACILLUS STEAROTHERMOPHILUS - PRODUCERS OF XYLANASE, METHOD OF XYLANASE PRODUCING AND XYLANASE ISOLATED FROM THE STRAINS OF BACTERIA BACILLUS STEAROTHERMOPHILUS SHOWING DELIGNIFICATION ACTIVITY Russian patent published in 1996 - IPC

FIELD: biotechnology. SUBSTANCE: proposed strains of bacteria - B. stearothermophilus NCIMB 40221 and NCIMB 40222 as producers of xylanase were selected from waters after treatment in cellulose and pulp plant Korsnas, Gavle, Sweden. Method involves the cultivation of strain-producers indicated above on the nutrient medium containing the nutrient sources that are necessary for biosynthesis, under aerobic condition, separation from the cultural fluid by centrifugation and precipitation by ammonium sulfate from the clarified broth followed by resuspension in the liquid medium of partially purified fraction of xylanase showing activity 15-20 U/ml or 35-40 U/ml, respectively, and preparing highly purified xylanase at activity 15 U/ml and 45 U/ml from the clarified cultural broth with simultaneous purification and concentration on cation-exchanger resin SE-52 or CM-52. Isolated xylanase showing delignification activity shows thermal stability at least at 65 C and pH=9, molecular mass 41000-42000 Da and partially determined N-terminal amino acid sequence. EFFECT: improved method of isolation of enzyme. 8 cl, 25 tbl