STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′-CC(A/T)-GG-3′ Russian patent published in 1995 - IPC

Abstract RU 2038380 C1

FIELD: microbiology, biotechnology. SUBSTANCE: new strain of bacterium Bacillus stearothermophilus ВКПМ B-5465 is proposed a producer of restriction endonuclease which can recognize and cleave nucleotide sequence 5′-CC(A/T)-GG-3′. Strain is isolated from thermal water sources as a result of restrictase producer screening. The yield of enzyme is 100000 U/g wet biomass, specific activity of the end enzyme is 30000 U/ml. Restrictase Bst 381 is an isoschizomer of restrictase Bst GII and can replace the latter in all genetic engineering studies. EFFECT: isolation of enzyme indicated above, increased specific activity. 1 dwg

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RU 2 038 380 C1

Authors

Repin Vladimir Evgen'Evich

Dates

1995-06-27—Published

1992-06-15—Filed