FIELD: medicine. SUBSTANCE: method involves the treatment of milled ovaries with acetic acid at pH 2.5-4.0 for 24-36 hr at 0-4 C with addition of zinc chloride (0.3-0.7 g/l), precipitation of polypeptides with acetone (8-12 volumes per 1 volume extract) at (-2)-(-8) C for 10-16 hr followed by separation of precipitate by filtration, drying, precipitate dissolving in distilled water, filtration and lyophilization. EFFECT: increased specific activity of product. 11 tbl
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Authors
Dates
1996-03-27—Published
1992-04-24—Filed