FIELD: medicinal microbiology, biochemistry. SUBSTANCE: the strain K. pneumoniae ГИСК N 214 is isolated from sick child feces with diarrhea. DNase is obtained by strain growing on liquid or solid nutrient medium, biomass sedimentation, ultrasonic disruption and centrifugation at 8000 rev/min for 30 min. Supernatant contains DNase. DNase activity is determined by plaque sowing on the solid nutrient medium containing DNA followed by incubation at 37 C for 18-24 hr and result recording. The presence of clear zone on the nutrient medium around plaques shows DNase activity. EFFECT: isolation of strain indicated above. 4 tbl
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