FIELD: biotechnology, genetic engineering. SUBSTANCE: invention proposes recombinant plasmid pThy 315 that can be used for preparing hybrid protein tumor necrosis α--factor - thymosin-α1 in bacterial cells. Molecular mass of plasmid - 2.1 MDa, it contains fragment of plasmid pThy 314 (size is 3.23 thousand nucleotide pairs) with early promoters tandem A2 and A3 of bacteriophage T7, ribosome binding site, gene of above indicated hybrid protein and transcription terminator of phage α, gene of a1-lactamase as genetic marker and unique restriction sites at the following positions (thousand nucleotide pairs): Eco RI - 0 and 0.11; BglIII - 0.78; ClaI - 0.27 and 0.73; Eco 47 III - 0.66; Eco 9 II - 0.65; KpnI - 0.25; PstI - 2.48, and XbaI - 0.83 and 0.93. EFFECT: plasmid indicated above. 2 dwg
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