FIELD: biotechnology, molecular biology, genetic engineering. SUBSTANCE: invention relates to preparing agents for plant protection with respect to coleopterous insects based on recombinant gram-negative bacteria. Recombinant plasmid DNA pBT 11 determining synthesis of crystalline insecticide delta-endotoxin Cry IIIA-type is constructed. Based on this plasmid DNA the strain of bacterium Pseudomonas putida containing above indicated plasmid and producing delta-endotoxin Cry IIIA-type showing activity with respect to insects of Coleoptera order is obtained. EFFECT: improved method of plasmid construction. 3 cl, 3 dwg
Authors
Dates
1998-01-27—Published
1995-11-23—Filed