METHOD OF PREPARING AJMALINE CONJUGATE WITH BOVINE SERUM ALBUMIN Russian patent published in 1997 - IPC

Abstract RU 2088230 C1

FIELD: biochemistry, immunology. SUBSTANCE: bovine serum albumin is activated preliminary with glutaric aldehyde at pH = 9.5 for 2 h. Glutaric aldehyde excess is removed by dialysis and ajmaline is added at ratio = 1:30 and dialyzed in 2 h against phosphate-saline buffer, pH = 7.3. Titer of the obtained serum is 1:4096, crossing binding with ajmaline analogs is 0-1.5% and that with 17-O-acetylajmaline - 87%. Ajmaline conjugate is used for preparing antibodies raised to ajmaline and for medicinal monitoring in cardiology. EFFECT: improved method of preparing. 1 tbl

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RU 2 088 230 C1

Authors

Babkina S.S.

Vinter V.G.

Zajnullina A.S.

Dates

1997-08-27—Published

1993-07-27—Filed