METHOD TO DETERMINE NEUTROPHIL CAPACITY TO PRODUCE ACTIVATED OXYGEN METABOLITES IN EXPERIMENT Russian patent published in 1997 - IPC

Abstract RU 2089907 C1

FIELD: medicine, diagnostics. SUBSTANCE: blood samples are taken, neutrophils are incubated in two parallel samples in a solution to maintain their viability with inductor-microorganisms of respiratory burst at the ratio of 1:100 and 1:10 at the presence of luminol, chemiluminescence is registered along with light total, maximal intensity and time for the onset of maximal luminescence of every sample at 1:100 ratio of neutrophil/microbe in reaction cuvette to analogous value at 1:10 ratio. Free-radical potential of neutrophils is calculated by a certain formula, at its value of 22-53 normal capacity is established for neutrophils to produce activated oxygen metabolites, at the level of 21-6 capacity is beneath the lower normal boundary and under the value of 6 insufficient capacity is stated on. EFFECT: higher information content, simplicity and efficiency at minimal expenses. 1 tbl, 1 dwg

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RU 2 089 907 C1

Authors

Sidorkin V.G.

Starikova M.A.

Bushuev Ju.I.

Pylaeva S.I.

Gordinskaja N.A.

Dates

1997-09-10Published

1993-06-08Filed