METHOD OF PREPARING THE CHOLESTEROL PHOSPHOLIPID CARRIER Russian patent published in 1997 - IPC

FIELD: medicine, biochemistry. SUBSTANCE: invention relates to technology of preparing the liposomal phospholipid carrier of cholesterol. Carrier is liposomal suspension and can interact selectively with human plasma blood lipoprotein of low density and can be used for the directed delivery of cholesterol in peripheral organs and tissues of organism. Method of preparing involves homogenization on plunger homogenizer under high pressure at optimal technological parameters. Method involves dissolving phospholipid and cholesterol in organic solvent, stirring, evaporation and drying followed by hydration, homogenization under high pressure by continuous process, centrifugation of liposomal suspension and sterilizing filtration. The dissolved cholesterol and phospholipids taken at molar ratio = (1:1)-(2:1) were filtered under pressure of inert gas and after evaporation and hydration subjected for cyclic homogenization in the closed contour under pressure 10-120 MPa, at 15-45 C, mainly near the point of phase transition of the corresponding phospholipid, at homogenization cycle number 1-20. Centrifugation is carried out at 1200 rev/min for 40 min, at +5 C. Molar ratio cholesterol/phospholipid in liposomal suspension = (0.9-1.1):(1.5-1.85), mean particle size is 40-200 nm, standard deviation of particles distribution is 20-100 nm. Method involves the use of egg yolk phospholipids and lecithin-enriched fraction of soybeans. Total concentration of phospholipids and cholesterol in liposomal suspension is 1-10 weight/vol.%. Invention can be used in medicine for preparing highly dispersed liposomal preparations showing cytostatic effect on tumors. Also, invention can be used in experimental biology, medicine, in part, in oncology and immunology for the directed delivery of cholesterol to organism tissues. EFFECT: increased output of process. 2 cl, 5 dwg