SOLID DIAGNOSTIC NUTRIENT MEDIUM AND A METHOD OF ITS PREPARING Russian patent published in 1998 - IPC

FIELD: applied biotechnology, microbiology. SUBSTANCE: solid nutrient medium used for qualitative and quantitative analysis of enterobacteria has nitrogen source, lactose, mannitol, mineral salts, dyes (neutral red and bromothymol blue) and water. The following mineral salts were used, %: NaCl 30-50; Na₂HPO₄×12H₂O 5-6; (NH₄)₂SO₄ 10-15; K₂SO₄ 25-40; MgSO₄×7H₂O 5-6, and Na₂CO₃ 2-3. Components were taken at the following ratio, g/l: mineral salts 7.5-9.5; lactose 8-12; mannitol 0.3-0.7; neutral red 0.03-0.05; bromothymol blue 0.01-0.03; agar-agar 5-10, and water - the rest. Method of medium preparing involves agar mixing with dyes and mineral salts. Then the obtained mixture is dissolved in distilled water, boiled, filtered, pH value is brought about to 7.0-7.2, sterilized and mixed with preliminary sterilized lactose and mannitol solution at pH = 7.0-7.2. EFFECT: high quality of analysis, decreased analysis time. 3 cl, 6 tbl