METHOD FOR PREPARING NUTRIENT MEDIUM FOR DETECTION OF COLON BACILLUS OF SEROTYPE O157 Russian patent published in 2006 - IPC C12N1/20 C12Q1/04 

FIELD: biotechnology, microbiology.

SUBSTANCE: method involves mixing the following components, g/l: powdered fish pancreatic hydrolyzate, 22.0-27.0; sodium chloride, 4.0-6.0; agar, 14.5-15.5; sorbitol, 9.0-11.0, and distilled water, balance, up to 1 l. After heating and melting agar pH value is brought about to 7.2 ± 0.2, and 18-22 ml of indicator is added to the prepared medium consisting, g/l: acid fuchsine, 4.5-5.5; bromothymol blue, 3.5-4.5; sodium hydroxide (1% solution), 150.0-170.0 ml, and distilled water, balance, up to 1l. Acid fuchsine is dissolved preliminary in 1% sodium hydroxide solution and bromothymol blue and distilled water are added to the prepared solution. Invention provides enhancing clearness of change colonies color in differential medium, decreasing incubation time and prolonged storage of medium ready for using. Invention can be used in clinical and epidemiological studies.

EFFECT: improved preparing method, improved and valuable properties of medium.

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