NUTRIENT MEDIUM FOR FLAX ANTHERS CULTURING Russian patent published in 1998 - IPC

Abstract RU 2120741 C1

FIELD: biotechnology. SUBSTANCE: invention proposes a medium containing the following components, mg/l: calcium chloride dihydrate, 435; potassium dihydrogen phosphate, 170; potassium nitrate, 1900; magnesium sulfate heptahydrate, 370; ammonium nitrate, 1650; iron sulfate heptahydrate, 27.8; ethylenediaminetetraacetate sodium monohydrate, 37.3; cobalt chloride hexahydrate, 0.025; copper sulfate pentahydrate, 0.025; boric acid, 6.2; potassium iodide, 0.75; manganese sulfate monohydrate, 22.3; sodium molybdate dihydrate, 0.25; zinc sulfate heptahydrate, 8.6; meso-inositol, 100; thiamine, 0.1; glutamine, 25; calcium pantothenate, 4.0; nicotinic acid, 0.5; pyridoxine, 0.5; asparagine, 250; glycine, 2.0; serine, 125; sucrose, 50 000; agar-agar, 7 000; 6-benzyladenine, 1.0; naphthylacetic acid, 0.5; indolylacetic acid, 0.3; and bidistilled water, the balance up to 1 l. Medium can be used in flax selection. The use of this nutrient medium provides increase of callusogenesis by 2-fold as compared with the known ones. EFFECT: enhanced effectiveness of medium. 1 tbl, 2 ex

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RU 2 120 741 C1

Authors

Poljakov A.V.

Proletova N.V.

Dates

1998-10-27Published

1996-06-27Filed