FIELD: biology. SUBSTANCE: method involves administration to eucaryotic cells population a nucleotide sequence encoding, for example, an enzyme mannoso-6-phosphate isomerase when nutrient medium has a compound that exhibits a toxic or inhibiting effect on cell growth, for example, mannose. An addition of reagent decreasing toxicity or inhibition effect ensures to identify and select transformed cells without any danger to kill nontransformed cells in population and without combined insertion of genes that determine resistance to antibiotics and herbicides ("positive selection"). Positive selection simplifies also vector structure used since one and the same gene can be used as both gene-reporter and selective gene. EFFECT: improved method of identification and selection. 9 cl, 4 dwg, 9 tbl, 12 ex
