FIELD: microbiology. SUBSTANCE: invention aims at revealing and isolating peptides fixed by means of bacterial adhesion to elastic epithelial tissues of mammary gland that can be used in vaccines for prevention of mastitis of agricultural animals. In order to reveal specific proteins in bacteria, e.g. in strain aureus, capable of sticking or not sticking to elastic epithelial cells, walls of bacterial cells are isolated and extracted. Proteins present in adhering cells and missing in other cells are used for preparing peptide subfragment. This peptide constitutes base of mastitis vaccine. EFFECT: provided effective mastitis vaccine blocking adhesion of strain aureus to elastic epithelial cells. 8 cl, 1 tbl, 8 ex
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