METHOD OF CONCENTRATION OF GLUCOAMYLASE FROM FUNGAL CULTURE OF ASPERGILLUS AWAMORI (STRAIN 120-77) Russian patent published in 2001 - IPC

Abstract RU 2163264 C2

FIELD: biotechnology, biochemistry, enzymology. SUBSTANCE: invention relates to processes of isolation and concentration of enzymes from cultural fluid. Method involves the combination of ultra- -filtration and vacuum-evaporation in unit of separating type at temperature of heat-carrier at inlet into unit 100 C, temperature of concentrate 45 C and under vacuum 0.09 MPa from filtrate of Aspergillus awamori (strain 120-77) cultural fluid. Obtained highly concentrated enzyme preparation has activity of glucoamylase 1500 U/ml. Preparation is stable at storage at temperatures from 18 to 25 C. Method ensures to prepare highly concentrated liquid enzyme preparation showing glucoamylase activity in industrial large-scale. Invention can be used in production of enzyme preparations for alcohol and starch-molasses industry. EFFECT: improved method of concentration, high activity of enzyme. 1 tbl, 2 ex

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RU 2 163 264 C2

Authors

Vasil'Ev A.E.

Safonov V.S.

Isaev A.S.

Ogorel'Tsev B.D.

Agafonov E.L.

Dates

2001-02-20—Published

1999-03-02—Filed