METHOD OF ESTIMATION OF IMMUNOGENICITY OF MICROORGANISM ANTIGENS Russian patent published in 2001 - IPC

FIELD: microbiology, mycology. SUBSTANCE: method of estimation of microorganism antigens immunogenicity involves mixing antigens with guinea pig complement, addition of rabbit stabilized blood, mixture incubation, smears preparing, selection of fixed leukocyte counts to be analyzed and determination of agglutinated (agglomerated) leukocytes among their. Results are evaluated by agglomeration index value as a ratio of agglutinated leukocytes to the analyzed amount of leukocytes. Cytoplasmic fraction of fungi of genus Candida, Aspergillus and Mucor are used as antigens of microorganisms. After incubation of mixture smear is fixed with methyl alcohol additionally and analyzed amount of leukocytes is selected to be 100. Antigen as a cytoplasmic fraction of fungus of genus Candida is considered to be immunogenic at value of agglomeration index 6-10%. Antigen as a cytoplasmic fraction of fungus of genus Mucor is considered to be immunogenic at leukocyte agglomeration index value 6-15%. Invention can be used for optimization of control of culturing process of fungi strains and immunological estimation of antigens. EFFECT: simplified and low-priced method of estimation of immunogenicity of soluble antigens of fungi of genus Candida, Aspergillus and Mucor. 3 tbl, 1 ex _