METHOD FOR DETERMINING PEROXIDASE ACTIVITY IN BIOLOGICAL FLUIDS Russian patent published in 2002 - IPC

Abstract RU 2180114 C1

FIELD: medicine. SUBSTANCE: method involves pouring biological fluid under study into the cells of two flat bottom 96-celled palettes. One cell is filled with physiological salt solution instead of the biological fluid and substrate mixture for peroxidase is added into the other cells of the first palette in use. Chromogen solution containing no hydrogen peroxide is added into the cells of the second palette and then, 50 mcl of 10% sulfuric acid is added 5-10 min later. Color intensity is measured in palette photometer at 492 nm wavelength. EFFECT: enhanced accuracy in determining functional activity of extracellular peroxidases with free radical influence being taken into account. 1 tbl

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RU 2 180 114 C1

Authors

Aznabaeva L.F.

Kil'Senbaeva F.A.

Aref'Eva N.A.

Dates

2002-02-27—Published

2000-10-13—Filed