METHOD OF ASSAY OF ANTIBODY RAISED TO CHICKEN REOVIRAL TENOSYNOVITIS ANTIGEN Russian patent published in 2002 - IPC

FIELD: veterinary virology, biotechnology. SUBSTANCE: method involves preparing virus-containing material from the strain "VNIVIP-DEP" and the following purification of virus-containing material is carried out by method of molecular-sieve chromatography on macroporous glass with pore diameter corresponding to diameter of virus virion to be purified. The best result is obtained using macroporous glass modified with polyvinylpyrrolidone with pore diameter 1200 , not less. Then method involves adsorption of highly purified antigen in the protein concentration 5-10 mcg/0.1 cm³, not less. Antispecies immunoperoxidase conjugate raised against to chicken IgG is used as conjugate. In carrying out spectrophotometric recording results in assay of final titer of sample to be analyzed by a single dilution 1:100 the following equation is used: lg titer = 2.19 x (LgS/P) + 3.59 where S/P = (S-NKx)/(PKx + NKx); S means value of optical density of analyzed serum; P means value of optical density of positive control; NKx means an average value of optical density of negative control; PKx means an average value of optical density of positive control. Invention can be used for determination of specific antibodies raised to chicken tenosynovitis reovirus by method of immunoenzyme analysis. EFFECT: enhanced sensitivity and specificity of analysis. 4 cl, 2 tbl