METHOD OF DIFFERENTIATION OF LISTERIA MONOCYTOGENES CULTURE Russian patent published in 2003 - IPC

FIELD: microbiology. SUBSTANCE: as species Listeria monocytogenes is a single species of genus Listeria with pathogenic properties for humans and animals it is necessary to differentiate this species from relates ones exactly. Method of differentiation of L. monocytogenes involves hydrolysis of lecithin in nutrient medium with addition of activated carbon and without addition. Method involves addition of 5 ml of 10% yolk egg suspension in physiological solution to 100 ml of medium GRM-1 (Obolenks) and lecithin hydrolysis is assayed in the presence of 0.5% of activated carbon but without additional supplements. L. monocytogenes shows hydrolytic activity with respect to lecithin in the presence of activated carbon only in contrast to other species of listeria. Method is specific, rapid and low-cost. Invention can be used for sanitary-epidemiological control of foodstuffs infectivity in centers of State sanitary and epidemiology inspection and food industry. EFFECT: improved method of culture differentiation. 2 tbl