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2 812 147

(13)

(51)

IPC

C12Q1/04(2006-01-01)

(21) (22)

Application

2023109311, 2023-04-12

(24)

Start date

2023-04-12

(22)

Actual filing date

2023-04-12

(45)

Published

2024-01-23

(72)

Inventor

Chalenko Iaroslava MikhailovnaKalinin Egor ValerevichErmolaeva Svetlana Aleksandrovna

(73)

Holder

Chalenko Iaroslava MikhailovnaKalinin Egor ValerevichErmolaeva Svetlana Aleksandrovna

METHOD OF DIFFERENTIATING LISTERIA MONOCYTOGENES FROM OTHER LISTERIA SPP. DOT BLOT METHOD USING CONJUGATED ANTIBODIES AGAINST INLB PATHOGENICITY FACTOR Russian patent published in 2024 - IPC C12Q1/04

Abstract RU 2812147 C1

FIELD: microbiology; biotechnology.

SUBSTANCE: method of identifying and differentiating Listeria monocytogenes is described. Microorganisms are grown on an agar nutrient medium containing 0.02% activated carbon. Imprints of colonies are obtained on a nitrocellulose membrane. Colony fingerprints are visualized using polyclonal monospecific antibodies against internalin B conjugated to horseradish peroxidase. The invention can be used for sanitary and epidemiological control of food contamination.

EFFECT: invention makes it possible to differentiate Listeria monocytogenes colonies from other microorganisms and representatives of the genus Listeria.

7 cl, 5 dwg, 1 tbl, 6 ex

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RU 2 812 147 C1

Authors

Chalenko Iaroslava Mikhailovna

Kalinin Egor Valerevich

Ermolaeva Svetlana Aleksandrovna

Dates

2024-01-23—Published

2023-04-12—Filed