FIELD: medicine, veterinary and food processing industry.
SUBSTANCE: claimed method includes manufacturing of corn-shell-type latex, wherein corn represents polystyrene latex and shell represents styrene-zinc methacrylate copolymer latex in repeating unit mass ratio of 1:0.8-1:0.4. Then latex is sensitized with monoclonal antibodies of lgG2a and lgG2b fractions in 0.01 M glycine buffer at pH 7.2-8.8 without preliminary conditioning followed by incubation primarily at 37°C for 130-150 min and further at 8°C for 18 hours. Obtained diagnosticum has specificity of 97±0.6, sensibility up to 10 pg/ml and makes it possible to obtain investigation results for 10 min or less.
EFFECT: accelerated method for diagnosticum production.
3 tbl
Authors
Dates
2005-12-20—Published
2003-07-17—Filed