LEGIONELLA DIAGNOSTICUM AND METHOD FOR PRODUCING IT Russian patent published in 1998 - IPC

FIELD: medicine. SUBSTANCE: diagnosticum has carrier and sensitine. Polystyrene latex with activated amine groups is used as carrier dyed with atraquinone blue soluble in fat, particle size being 0.804 mcm, variation coefficient being equal to 4.1-4.5%, variance coefficient being equal to 1.003-1.006 and amine group concentration being et 2.5 2,5•10⁴-10⁵ mole/g. Group-specific protein fraction of L. pneumophila Philadelphia 1 (serologic group 1) external membrane antigens having molecular mass 45 kD. Method involves incubating 1% suspension of preliminarily washed particles used as polystyrene latex carriers with solution of group-specific protein fraction used as sensitine having protein concentration of 150 mcg/l with volume ratio of 1:1. Latex particles are treated with glycine buffer having 7.8 pH and reincubated. The so obtained diagnosticum preparation is 0.5% latex particles suspension covalent bound to group-specific protein fraction. EFFECT: high accuracy and specificity of the method.