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2 271 010

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IPC

G01N33/577(2006-01-01)

A61K39/395(2006-01-01)

C12N5/20(2006-01-01)

C12P21/08(2006-01-01)

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Application

2004109560/15, 2004-03-31

(24)

Start date

2004-03-31

(22)

Actual filing date

2004-03-31

(45)

Published

2006-02-27

(72)

Inventor

Vlasik Tat'Jana NikolaevnaMazurov Aleksej VladimirovichKhaspekova Svetlana GeorgievnaJanushevskaja Elena Vadimovna

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju Mona"Gosudarstvennoe Uchrezhdenie Rossijskij Kardiologicheskij Nauchno-Proizvodstvennyj Kompleks Ministerstva Zdravookhranenija Rf Mz Rf)

METHOD FOR IMMUNOENZYME ANALYSIS FOR ASSAY OF VON WILLEBRAND FACTOR, MONOCLONAL ANTIBODY TO VON WILLEBRAND FACTOR (VARIANTS) AND STRAIN OF HYBRID CULTURED MAMMAL CELLS MUS MUSCULUS L - PRODUCER OF MONOCLONAL ANTIBODIES TO VON WILLEBRAND FACTOR (VARIANTS) Russian patent published in 2006 - IPC G01N33/577 A61K39/395 C12N5/20 C12P21/08

Abstract RU 2271010 C2

FIELD: immunology.

SUBSTANCE: invention relates to immunoenzyme analysis and can be used for assay of von Willebrand factor. Method involves immunoenzyme analysis wherein monoclonal antibody 5C3 is used as an immobilizing antibody, and a mixture of biotin-labeled monoclonal antibodies 2H2 and 7D12 is used as a detecting antibody. Also, invention relates to monoclonal antibodies produced by the strain of hybridoma cultured cells Mus musculus L. and directed against von Willebrand factor, and to strains of hybrid cultured cells Mus musculus L. producing indicated monoclonal antibodies. Invention provides the development of highly sensitive method for assay of von Willebrand factor.

EFFECT: improved method for analysis.

9 cl, 1 tbl, 2 dwg, 3 ex

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RU 2 271 010 C2

Authors

Vlasik Tat'Jana Nikolaevna

Mazurov Aleksej Vladimirovich

Khaspekova Svetlana Georgievna

Janushevskaja Elena Vadimovna

Dates

2006-02-27—Published

2004-03-31—Filed