FIELD: chemistry; biochemistry.
SUBSTANCE: invention discloses a strain of hybrid animal cells Mus musculus L.4 A2, which is deposited in the Collection of cell cultures of the State Research Center of Virology and Biotechnology VECTOR, which is a producer of monoclonal antibodies which are specific to the matrix protein VP40 of the Ebola virus, Zaire subtype (Mainga strain), and a strain of hybrid animal cells Mus musculus L. 1C1 which is deposited in the Collection of cell cultures of the State Research Center of Virology and Biotechnology VECTOR, which is a producer of monoclonal antibodies which are specific to the matrix protein VP40 of the Ebola virus, Zaire subtype (Mainga strain). The invention is also aimed at obtaining monoclonal antibodies 4A2 which are produced by the 4A2 hybridome, (subclass of immunoglobulins IgGl which have a heavy 55 kDa and a light 25 kDa chain) and are used as binding antigens in the "sandwich" format immunoenzymometric system for exposing the matrix protein VP40 of the Ebola virus, Zaire subtype (Mainga strain), and monoclonal antibodies 1C1 produced by the 1C1 hybridome (subclass of immunoglobulins IgGl which have a heavy 55 kDa and a light 25 kDa chain), used as biotin labelled indicators in the "sandwich" format immunoenzymometric system for exposing the matrix protein VP40 of the Ebola virus, Zaire subtype (Mainga strain). The disclosed antibodies are used together in a "sandwich" format immunoenzymometric system for exposing the matrix protein VP40 of the Ebola virus, Zaire subtype (Mainga strain).
EFFECT: invention enables to obtain monoclonal antibodies which are specific and do not compete with each other for antigen epitopes and which, when used together in a "sandwich" format immunoenzymometric system, ensure high reliability of results for exposing the matrix protein VP40 of the Ebola virus.
5 cl, 3 dwg, 1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF HYBRID ANIMAL CELLS Mus museums L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING NUCLEOPROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC TEST SYSTEM FOR EXPOSING NUCLEOPROTEIN OF EBOLA VIRUS, ZAIRE SUBTYPE (Mainga STRAIN) | 2008 |
|
RU2395576C1 |
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|
RU2686630C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus L - PRODUCER OF MONOCLONAL ANTIBODIES FOR EXPOSING VP40 PROTEIN OF MARBURG VIRUS, (Popp STRAIN) (VERSIONS), MONOCLONAL ANTIBODY PRODUCED BY SAID STRAIN (VERSIONS) AND SET FOR "SANDWICH" FORMAT IMMUNOENZYMOMETRIC SYSTEM FOR EXPOSING VP40 MATRIX PROTEIN OF MARBURG VIRUS (Popp STRAIN) | 2008 |
|
RU2395575C1 |
| MONOCLONAL ANTIBODY SPECIFIC FOR EBOLA VIRUS | 2018 |
|
RU2705763C1 |
| STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS L3F9 - PRODUCER OF MONOCLONAL ANTIBODIES SUITABLE FOR USE IN "SANDWITCH" IMMUNOENZYMOMETRIC SYSTEM FORMAT FOR DETECTING VP35 PROTEIN OF MARBURG VIRUS AND 3F9 MONOCLONAL ANTIBODIES PRODUCED BY SAID STRAIN OF HYBRID CELLS | 2008 |
|
RU2393220C1 |
| RECOMBITANT PLASMID DNA pET21-VP40VE CONTAINING THE GENE FOR THE MATRIX PROTEIN VP40 OF EBOLA VIRUS AND THE RECOMBITANT PROTEIN VP40-VE OBTAINED AS A RESULT OF THE GENE EXPRESSION FOR THE PROTEIN VP40 OF EBOLA VIRUS USING THE RECOMBITANT PLASMID DNA pET21-VP40VE AND EXHIBITING IMMUNOGENIC AND ANTIGENIC PROPERTIES | 2020 |
|
RU2742511C1 |
| RECOMBINANT PLASMID DNA PET21-NPVE CONTAINING NUCLEOLOPROTEIN (NP) GENE OF EBOLA VIRUS AND RECOMBINANT PROTEIN NP-VE, OBTAINED AS RESULT OF EXPRESSION OF NP GENE OF EBOLA VIRUS USING RECOMBINANT PLASMID DNA PET21-NPVE AND POSSESSING IMMUNOGENIC AND ANTIGENIC PROPERTIES | 2020 |
|
RU2739505C1 |
| HSOBIENS-WT/GIN/2015/KALIDIE-KINDIA-1022 STRAIN OF EBOLA ZAIRE TO OBTAIN ANTIGEN USED AS COMPONENT OF IMMUNOENZYMOMETRIC TEST SYSTEM FOR DETECTION OF G AND M ANTIBODIES TO VIRUS OF EBOLA | 2016 |
|
RU2631937C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus MUSCULUS 1F1 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID PROTEIN N OF THE SARS-CoV-2 VIRUS | 2021 |
|
RU2769817C1 |
| STRAIN OF EBOL VIRUS "ZAIR CH-5" FOR CARRYING OUT MODEL EXPERIMENTS AND PREPARING DIAGNOSTIC AND VACCINE PREPARATIONS | 2001 |
|
RU2225440C2 |
