FIELD: biotechnologies.
SUBSTANCE: invention is an elective nutrient medium, which may be used in laboratory practice for extraction of a cholera agent. The nutrient medium contains a nutrient base, microbiological agar, saccharose, sodium chloride, sodium carbonate, bismuth nitrate pentahydrate, sodium citrate, cattle bile, EDTA iron (III)-sodium salt, bromthymol blue, mesoinositol, activated charcoal, sodium sulfite and L-cysteine, potassium tellurite and distilled water. The nutrient base in the nutrient medium is a dry nutrient broth from a pancreatic overcook of Caspian sprat or a dry nutrient broth from hydrolysate of fish and bone flour or a complex of yeast extract and peptone at the specified ratio of components.
EFFECT: invention makes it possible to increase yield of choleraic vibrio and to increase differentiating properties of the medium.
11 tbl, 11 ex
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Authors
Dates
2013-06-10—Published
2012-05-03—Filed