PREPARATION FOR NORMALIZING FEMALE REPRODUCTIVE FUNCTION AND METHOD FOR ITS OBTAINING Russian patent published in 2007 - IPC A61K35/54 A61P15/08 

FIELD: medicine.

SUBSTANCE: the present innovation deals with isolating biologically active substance out of animals' ovaries to obtain medicinal form for parenteral intake that could be applied as a remedy to normalize female reproductive function. This preparation is being a peptide complex at the content of low-molecular fraction ranged 70-90%, at molecular weight of peptide components in it being within 70-712 Da, at concentration of polypeptides ranged 2.5÷2.9 mg/ml and obtained out of the ovaries of 12-mo-aged calves, not older or from swine due to extracting acetic acid in the presence of zinc chloride. The suggested technique for obtaining the preparation mentioned deals with the fact that ovaries from calves or swine's should be frozen at -40°C, not less to be kept at -20÷22°C for 2 mo, not less to be then reduced, 3%-acetic acid solution should be added at volumetric ratio of 1:5 at 20°±5°C; extraction should be fulfilled at constant mixing and on obtaining homogeneous suspension it should be supplemented with 1%-zinc chloride solution at volumetric ration of 7°÷16°C, then it is necessary to mix per 1 h in every 4 h keeping for 48 h, the extract should be separated against ballast substances due to separating, the extract should be supplemented with acetone at volumetric ratio of 1:5 to be kept at 3÷5°C for 4 h, the developed homogenized residue should be repeatedly precipitated with acetone twice, not less, then the residue that contains an active substance should be washed upon Nutsch filter with 2-fold volumes of acetone (cooled up to 7÷16°C) till obtaining the residue of light-gray color, grated through metallic sieve, dried, dissolved in distilled water at a room temperature and constant mixing up to polypeptides concentration being 2.5÷2.9 mg/ml,; the solution should be centrifuged, filtered and purified ultrafiltrationally upon the equipment at anti-pressure being not more than 1.0 kilogauss/sq. cm through materials at inhibiting capacity of 15000 Da, ultrafiltrate should be supplemented with glycocoll up to its final concentration being 10÷20 mg/ml at pH=5.6÷6.6, the solution should undergo sterilizing filtration at the pressure being not more than 2.0 kilogauss/sq. cm to be poured into ampoules per 2 ml and autoclaved for 8 min at 120°C and atmospheric pressure of 1.1 kilogauss/sq. cm. The innovation provides optimal technology to isolate peptide complex at the content of low-molecular fraction from 70 to 90% at molecular weight of peptide components in it ranged 70-712 Da and obtain water solution of the extract at concentration of polypeptides being 2.5÷2.9 mg/ml that enables not only purify the obtained product against admixtures but increase its output; and, also, the isolated substance is distinct against known ones obtained earlier out of animal ovaries by molecular weight of peptide complexes in it and by its nontoxicity and apyrogeneity due to complete purification against admixtures.

EFFECT: higher efficiency.

2 cl, 1 dwg, 5 ex, 2 tbl